Generation of Transgenic Mice

Generation of YAC transgenic mice by ICSI

Recombineering of BAC DNA for the Generation of Transgenic Mice

The use of BAC plasmids for transgenic gene expression is also gaining popularity over traditional proximal promoter driven transgene expression because the BAC typically contain most, if not all, of the important regulatory elements required to recapitulate endogenous gene expression (Giraldo and Montoliu 2001). Maintaining the coding sequence within its regulatory locus insulates the transgen...

Development of Transgenic Mice Harboring Ovine Beta Lactoglobulin-Calcitonin Transgene

Generation of transgenic mice with mild and severe retinal neovascularisation.

AIM To generate a mouse model for slow progressive retinal neovascularisation through vascular endothelial growth factor (VEGF) upregulation. METHODS Transgenic mice were generated via microinjection of a DNA construct containing the human VEGF165 (hVEGF) gene driven by a truncated mouse rhodopsin promoter. Mouse eyes were characterised clinically and histologically and ocular hVEGF levels as...

Next-generation transgenic mice for optogenetic analysis of neural circuits

Here we characterize several new lines of transgenic mice useful for optogenetic analysis of brain circuit function. These mice express optogenetic probes, such as enhanced halorhodopsin or several different versions of channelrhodopsins, behind various neuron-specific promoters. These mice permit photoinhibition or photostimulation both in vitro and in vivo. Our results also reveal the importa...